a549 ace2  (ATCC)

Journal: ACS Omega

Article Title: A Tetrapodal Tryptophan Derivative with Multiple Exposed Free Carboxylic Acids Blocks Host Cell Entry of Omicron SARS-Cov-2 and Respiratory Syncytial Virus

doi: 10.1021/acsomega.5c10442

Figure Lengend Snippet: Evaluation of the antiviral activity and toxicity of different compounds of our in-house collection against VSV pseudotyped with BA.2 (A) and BA.4/5 S (B) proteins. VSV pseudotyped with the indicated S proteins was preincubated with the different compounds at 20 μM, followed by the infection of A549-ACE2-TMPRSS2 cells. Viral infection was monitored by counting GFP-expressing cells. Toxicity was assessed via the analysis of cell confluency. Bars indicate the mean and SE for n = 3. Individual data points are shown as circles.

Article Snippet: A549-ACE2-TMPRSS2 cells (catalog a549-hace2tpsa) were purchased from InVivoGen.

Techniques: Activity Assay, Infection, Expressing

Journal: ACS Omega

Article Title: A Tetrapodal Tryptophan Derivative with Multiple Exposed Free Carboxylic Acids Blocks Host Cell Entry of Omicron SARS-Cov-2 and Respiratory Syncytial Virus

doi: 10.1021/acsomega.5c10442

Figure Lengend Snippet: Compound 2 blocks the entry of RSV into cells. Time-of-addition experiment with 2 treatment at 4 μM prior to and during infection ( Preincubation and Pre - + Post-incubation ) or following infection ( Post-incubation ) of A549 cells with RSV-A encoding the fluorescent protein mKate2 (see ). Viral infection was quantified at 24 h post infection by examination of mKate2 fluorescence.

Article Snippet: A549-ACE2-TMPRSS2 cells (catalog a549-hace2tpsa) were purchased from InVivoGen.

Techniques: Infection, Incubation, Fluorescence

Journal: ACS Omega

Article Title: Exploring SARS-CoV‑2 Spike RBD Pockets as Targets for Generic Drugs: A Combined Computational, Biophysical, and Biological Approach

doi: 10.1021/acsomega.5c05175

Figure Lengend Snippet: In vitro antiviral activity of selected compounds. (A) Antiviral activity against VSV pseudotyped with the S protein of Wuhan-Hu-1 and cellular toxicity for the indicated compounds in VeroE6 cells ( n = 3). (B) Virus production of SARS-CoV-2 infection by the indicated compounds at 20 μM or remdesivir (30 μM) in either A549-ACE2 or VeroE6-TMPRSS2 cells. (C) Antiviral activity of fingolimod against VSV pseudotyped with the indicated S protein ( n = 3). (D) Concentration reducing 50% of viral infection of VSV pseudotyped with the indicated S protein. All data represent the mean and standard error of 3 independent replicates. * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001 by two-tailed t test on log-transformed data.

Article Snippet: VeroE6-TMPRSS2 (Catalog No. JCRB1819, Japanese Collection of Research Bioresources), VeroE6 (kindly provided by Dr. Luis Enjuanes, CNB–CSIC, Spain), and A549-Ace2-TMPRSS2 (Catalog No. a549-hace2tpsa, Invivogen) were cultured in DMEM high glucose, with glutamine, 10% FBS, and 1% penicillin/streptomycin.

Techniques: In Vitro, Activity Assay, Virus, Infection, Concentration Assay, Two Tailed Test, Transformation Assay